Browsing by Author "Mtui, Godliving"

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Extracellular enzymes from brown-rot fungus Laetiporus sulphureus isolated from mangrove forests of coastal Tanzania
    (2008) Mtui, Godliving; Masalu, Rose
    A brown-rot fungus, Laetiporus sulphureus (Fr.) Murr., was isolated from Mbweni, Oyster Bay and Mtoni Mangove Forests in Dar es Salaam, Tanzania, and the biochemical properties of its extracellular enzymes were investigated. The crude culture filtrate was concentrated by ultrafiltration. Protein content and lignocellololytic enzyme activities were measured by photometric methods. The crude enzyme extract was purified by gel chromatography and characterized by sodium docecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The fungal filtrate had maximum manganese peroxidase (MnP) of 2.5 U/mL and lignin peroxidase (LiP) of 1 U/mL, but showed no laccase (Lac) activity. The enzyme extracts were able to oxidize rhemazol brilliant blue-R (RBB-R) dye and phenol, and could remove up to 90% color from raw textile effluent in immobilized culture. The purified peroxidases showed that the MnP from S. sulphureus has a molecular weight of 48 kDA. The study elucidated the extracellular enzymes profile of facultative marine L. sulphureus and provided basic information on their potential for biological wastewater treatment systems
  • Thumbnail Image
    Item
    Optimization of Laccase and Manganese Peroxidase Production in Submerged Culture of Pleurotus Sajor - caju
    (African Journal of Biotechnology, 2011-07-08) Patrick ,Ferdinandi; Mtui, Godliving; Mshandete ,Anthony Manoni; Kivaisi, Amelia
    A white-rot fungus, Pleurotus sajor-caju, was isolated from coastal Tanzania and screened for crude lignolytic enzymes production using rhemazol brilliant blue R (RBBR) dye, 2,2-azino-bis (3 ethylbenzthiazoline)-6-sulfonate (ABTS) and guaiacol in a semi-solid medium. Laccase (Lac) and manganese peroxidase (MnP) were detected by α αα α-napthol and pyrogallol solutions, respectively, on the guaiacol supplemented semi-solid media. The effect of temperature, pH, carbon, nitrogen, Cu2+, 2,5 xylidine, ferulic acid, Mn2+ and immobilization using Luffa cylindrica sponges in submerged culture fermentations were investigated for maximum enzymes production. After 7 days of incubation, 83 to 100% oxidation of RBBR, ABTS and guaiacol was observed. With optimized culture conditions, the fungal filtrate had maximum Lac and MnP activities of 80 and 0.94 U/ml, respectively compared to 0.62 and 0.0003 U/ml obtained with non-optimized ones; amounting to 129 and 3133 times increase in Lac and MnP activities, respectively. The improved crude enzymes activities, RBBR decolourization, ABTS and guaiacol oxidation capabilities of P. sajor-caju show its potential as a source of industrial enzymes for biotechnological applications