Browsing by Author "NYANGI,CHACHA"

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Aflatoxin and Fumonisin Contamination of at-harvest and Storage Beans in Babati District, Northern Tanzania
(Greener Journal of Agricultural Sciences, 2016-11) NYANGI,CHACHA
The natural occurrence of total aflatoxin and fumonisin was determined in 38 bean at-harvest samples and 106 storage beans samples in the year 2013 in Babati District Northern Tanzania. Quantification for total aflatoxin and fumonisin was done using Enzymes Linked Immunosorbent Assay - ELISA (Reveal AccuScan® Neogen, USA), and the results were confirmed using Liquid Chromatography Tandem Mass Spectrometer (LC-MS/MS). Eighteen percent (7/38) of bean at harvest samples were contaminated with up to 3 µg/kg aflatoxins and no bean sample was contaminated with fumonisins. There was a significant correlation (p < 0.05) between the concentration of aflatoxins and fumonisins with climatic zones and agronomic practices. Only bean samples from the high-altitude high rain zone were contaminated with aflatoxins (mean level of 1.53 µg/kg). For storage beans, samples from Seloto village were associated with higher aflatoxin concentration (mean of 3.74 µg/kg) and those from long village were associated with higher fumonisin levels (mean of 9.0 mg/kg). These results indicate that beans consumers in the study area are exposed to the danger of chronic exposure to aflatoxin and fumonisin poisoning. Thus, those practices that reduce contamination should be adopted by all farmers in the study area to reduce the health hazards associated with consumption of contaminated beans. This also calls for further research to investigate human exposure to aflatoxin and fumonisin in the study area.