Browsing by Author "Wang, Yangcai"

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    Enhanced Aquaculture Wastewater Treatment Using Water Spinach (Ipomoea Aquatica Forsskal) and Exogenous Compound Bacteria
    (Elsevier, 2024) Xu, Jiaojiao; Nicholaus, Regan; Wang, Yangcai; Yang, Wen; Zhu, Jinyong; Zheng, Zhongming
    Bioremediation technology has been widely recognized as a sustainable treatment method for purifying aqua culture wastewater. We established two types of ecological floating beds, one with water spinach (WS) only and another with water spinach combined with exogenous compound bacteria (WS-ECB) for the treatment of the Pacific white shrimp aquaculture wastewater of low-salinity. The exogenous compound bacteria used in this study was composed of Rhodopseudomonas palustris and Bacillus subtilis in a certain proportion. Microbial com munity dynamics were analyzed by using high-throughput sequencing of 16S rRNA and water quality was determined. Both two treatments were effective in the remediation of aquaculture wastewater. The removal efficiencies of CODMn, TN and TP in the WS group were 34.33 %, 66.54 % and 73.01 %, respectively, and the removal efficiency of NH4 + in the WS-ECB group was 93.79 %. Furthermore, the addition of water spinach and exogenous compound bacteria increased the abundance of some bacteria that are involved in regulating water quality and altered the succession of microbial communities. The microbial communities were shaped by both stochastic processes (including limited dispersal) and deterministic processes (including environmental condi tions and biotic interactions). Importantly, stochastic processes dominated the assembly of both water and rhizosphere microbial communities in different treatments, while deterministic processes contributed more to the rhizosphere microbial community in the WS-ECB than in the WS. Overall, our study emphasizes that plant and microbial remediation was a potential and effective approach to remove the nutrients of water, and clarifies the bacterial community dynamics and assembly mechanisms during aquaculture wastewater treatment processes.
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    The Compound Bacillus and Sea Purslane (Sesuvium Portulacastrum) Enhanced Aquaculture Wastewater Treatment Efficiency: Insights from a Study on Microbial Community Distributions.
    (ELSEVIER, 2025-03-31) Hongmei Yuan; Li, Junxian; Wang, Haihang; Nicholaus, Regan; Ramzan, Muhammad Naeem; Yang, Wen; Zheng, Zhongming; Wang, Yangcai
    As the aquaculture industry continues to grow rapidly, the environmental consequences of wastewater discharge from aquaculture have become a major concern. The biological treatment method has proven to be an effective and beneficial approach for wastewater treatment. In this experiment, aquaculture wastewater was treated using two different ratios of Bacillus subtilis and Bacillus licheniformis composites in combination with sea purslane (Sesuvium portulacastrum) to investigate the wastewater treatment efficiency and microbial community. Sea purslane effectively removed nutrients from aquaculture wastewater. The addition of compound Bacillus significantly increased the removal rates of CODMn, TP, and PO4 3− -P, which were 54.9–56.4 %, 78.2–79.9 %, and 85.0–89.6 %, respectively. Moreover, the compound Bacillus notably influenced the microbial community composition in the water and root systems, reducing species abundance while increasing community diversity, with Treatment 2 exhibiting the highest community stability. The addition of the compound Bacillus significantly increased the relative abundance of Alphaproteobacteria. Most of the important operational taxonomic units (OTUs) that significantly impacted the groups belonged to Alphaproteobacteria. Different biomarkers in the water and root system were primarily from Alphaproteobacteria, Gammaproteobacteria, and Bacteroidia, including Idiomarina and Alteromonas, Ruegeria, Vibrio, Algoriphagus, and Maribacter, among others. These microorganisms play a significant role in promoting the removal rate of nitrogen and phosphorus. Furthermore, the compound Bacillus notably affected the abundance of nitrogen cycle-related sequences and increased the relative abundance of microorganisms associated with the nitrogen cycle. These findings provide valuable insights for optimizing aquaculture wastewater treatment processes.