Browsing by Author "Wei, Yingzhen"

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    Exogenous Compound Bacteria Enhance the Nutrient Removal Efficiency of Integrated Bioremediation Systems: Functional Genes and Microorganisms Play Key Roles
    (ELSEVIER, 2024) Wei, Yingzhen; Zhu, Jinyong; Shen , Ding; Yang, Wen; Nichola, Regan; Wang , Yangcai; Zheng, Zhongming; Lukwambe, Betina
    With the continuous development of intensive mariculture, the application of the integrated bioremediation system of aquaculture wastewater (IBSAW) is increasingly promoted. However, the process and nutrients removal performance of the IBSAW need to be further optimized due to its immature technologies. In this study, exogenous compound bacteria (ECB) were added to IBSAW to investigate its pollutants removal efficiency and the relevant mechanisms. High-throughput sequencing and Geochip gene array were used to analyze the cor relation between nutrients and bacteria, and the abundance of N and P cycling genes were quantified. Multi variable statistics, dimensionality reduction analysis, and network analysis were applied to explore the mechanisms of IBSAW operation. The results showed that the nutrients decreased significantly after adding ECB, with the brush treatment group significantly outperforming the ceramsite in removing NO 3 has an advantage in removing NO 2 and PO 4 3 . Ceramsite -N. The addition of ECB and different substrates significantly affected the composition of bacterial communities. The contents of nosZ and nirKS related to denitrification in the treatment groups were significantly higher than those in the control group, and the contents in the brush treatment group were significantly higher than that of ceramsite. The biomarkers Psychroserpens and Ruegeria on the biofilm of the brush treatment group were positively correlated with nirKS, while Mycobacterium, Erythrobacter and Paracoccus, Pseudohaliea in the ceramsite group were positively correlated with nirS and nirK, respectively. Therefore, it is speculated that the ECB significantly promoted the increase of denitrification bacteria by affecting the compo sition of bacterial communities, and the ECB combined with functional genera improved the efficiency of nu trients removal in the system. This study provided a reference for understanding the process and mechanism of nutrients removal, optimizing the wastewater purification technology of the IBSAW and improving the perfor mance of the system.
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    Nitrogen and Phosphorus Related Functional Genes Enhance Nutrient Removal in The Integrated Aquaculture Wastewater Bioremediation System in The Presence of Photosynthetic Bacteria.
    (Springer, 2024-12-20) Ramzan, Muhammad Naeem; Shen, Ding; Wei, Yingzhen; Emmanuel, Arslan; Nicholaus, Regan; Yang, Wen; Zheng, Zhongming
    Integrated Aquaculture Wastewater Bioremediation Systems (IAWBSs) are crucial for treating nutrient-rich mariculture water. However, there is a lack of detailed information about the functional mechanisms between nitrogen (N) and phosphorus (P) functional genes and the bacterioplankton community in the presence of photosynthetic bacteria (PSB). This study evaluated the connections between N and P functional genes in IAWBSs under the influence of PSB. The results showed significant improvements in effluent quality, with removal efficiencies of 79, 74, 83, 90, and 71% for NO2 −−N,NO3 −−N, PO4 3−-P, NH4 +−N, and CODmn, respectively. Adding PSB enhanced and altered microbial diversity within the system, promoting the abundance of functional genes related to N and P cycling. Notably, genes associated in denitrification (nirK and nirS), ammonification (ureC) and amoB, involved in nitrification, were significantly increased after PSB was added. Furthermore, genes such as phnK, phoD, and phoX, which are involved in P transformation, also showed increased abundance levels. These genes were closely linked to the microbial community distribution, species diversity, and nutrient cycling. Microbial community changes can result in changes in functional gene abundance. This study provides important and novel insights for developing bioremediation strategies for polluted sites. This demonstrates the fundamental relationships between the IAWBSs’ functional units and the distribution of microbial communities under the influence of PSB.
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    Nitrogen and Phosphorus‑Related Functional Genes Enhance Nutrient Removal in the Integrated Aquaculture Wastewater Bioremediation System in the Presence of Photosynthetic Bacteria
    (Springer nature, 2025) Ramzan, Muhammad N.; Shen, Ding; Wei, Yingzhen; Emmanuel, Arslan; Nicholaus, Regan; Yang, Wen; Zheng, Zhongming
    Integrated Aquaculture Wastewater Bioremediation Systems (IAWBSs) are crucial for treating nutrient-rich mariculture water. However, there is a lack of detailed information about the functional mechanisms between nitrogen (N) and phosphorus (P) functional genes and the bacterioplankton community in the presence of photosynthetic bacteria (PSB). This study evaluated the connections between N and P functional genes in IAWBSs under the influence of PSB. The results showed significant improvements in effluent qual- ity, with removal efficiencies of 79, 74, 83, 90, and 71% for NO2− −N, NO3− −N, PO43−-P, NH 4+ −N, and COD Mn, respectively. Adding PSB enhanced and altered microbial diversity within the system, promoting the abundance of functional genes related to N and P cycling. Notably, genes associated in denitrification (nirK and nirS), ammonification (ureC) and amoB, involved in nitrification, were significantly increased after PSB was added. Fur- thermore, genes such as phnK, phoD, and phoX, which are involved in P transformation, also showed increased abundance levels. These genes were closely linked to the micro- bial community distribution, species diversity, and nutrient cycling. Microbial community changes can result in changes in functional gene abundance. This study provides important and novel insights for developing bioremediation strategies for polluted sites. This demon- strates the fundamental relationships between the IAWBSs’ functional units and the distri- bution of microbial communities under the influence of PSB.