Journal Articles

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Browsing Journal Articles by Author "Beed F."

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    Aflatoxins and Fumonisin Contamination of Marketed Maize, Maize Bran and Maize Used as Animal Feed in Northern Tanzania.
    (AFRICAN SCHOLARLY SCIENCE COMMUNICATIONS TRUST, 2016-07) Nyangi Chacha; Mugula J.K; Beed F.; Boni S.; Koyano E.; Sulyok, M.
    The objective of this study was to investigate the occurrence of total aflatoxin and total fumonisin in maize and maize-based products in Babati, northern Tanzania. A total of 160 samples were collected in 2013-14. Quantification for total aflatoxin and fumonisin was done using enzyme-linked immunosorbent assay (Reveal AccuScan® Neogen, USA) and the results were confirmed using Liquid Chromatography Tandem Mass Spectrometer. Aflatoxin was detected in 32% of maize samples (mean 3.4 ± 0.3 µg/kg; range 2.1–16.2 µg/kg) and fumonisin in 39% of samples (mean 5.6 ± 1.40 mg/kg; range 0.4–62.0 mg/kg). Among marketed products, maize bran (used for animal feed) was the most contaminated (2.4 µg/kg aflatoxin and 1 mg/kg fumonisin), followed by whole maize in market stalls (1.9 µg/kg aflatoxin and 0.4 mg/kg fumonisin) and then maize flour (1.4 µg/kg aflatoxin and 0.3 mg/kg fumonisin). Un-marketed maize sorted out by farmers as “bad” and intended for animal feeding was the most contaminated (overall mean aflatoxin and fumonisin levels of 1.7 µg/kg and 7.4 mg/kg, respectively). The results indicate that levels of aflatoxin and fumonisin contamination in marketed maize were within tolerable limits.
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    Assessment of Pre-Harvest Aflatoxin and Fumonisin Contamination of Maize in Babati District, Tanzania.
    (AFRICAN SCHOLARLY SCIENCE COMMUNICATIONS TRUST, 2016-07) Nyangi C.; Beed F.; Mugula J.K; Boni S.; Koyano E,; Mahuku G.; Sulyok M.; Bekunda M.
    A survey was conducted in 2013 to establish total aflatoxin and total fumonisin in maize, as well as farmers’ practices relating to maize cultivation and awareness of mycotoxins, in three villages of Babati District, northern Tanzania. Quantification of total aflatoxin and fumonisin was done using enzyme-linked immunosorbent assay (Reveal AccuScan® Neogen, USA) and the results were confirmed using Liquid Chromatography Tandem Mass Spectrometer. The mean aflatoxin was 2.94 µg/kg and all samples (n=440) were within the East African Community (EAC) standard of 10 µg/kg for total aflatoxin, but the mean fumonisin was 5.15 mg/kg, more than double the EAC standard of 2 mg/kg, and 35% of samples exceeded this standard. Maize samples obtained from farmers in the village in the mid altitude, dry zone had significantly higher mean aflatoxin (3.32 µg/kg) and significantly lower mean fumonisin (3.17 mg/kg) than maize from the other two villages (in the high and mid altitude, high rainfall zones). Most farmers (n=442) were male (72%), educated to primary school level (77%) and aware of mycotoxins (62%). As well as participating in a development program, Africa Research in Sustainable Intensification for the Next Generation, most (86%) farmers had experience of working with other development programs. All farmers used flat planting, most used improved seeds (98%), ox ploughing (78%), insecticides (78%) and early planting (36%). Practices associated with mycotoxins were planting time, tillage methods, previous season planted crops, and use of insecticides. Awareness of mycotoxins and climatic conditions were also associated with mycotoxin prevalence. In conclusion, good practices are associated with acceptable aflatoxin levels and should be continued. However, the high level of fumonisins warrants further investigation.