Browsing by Author "Zheng, Zhongming"

Now showing 1 - 6 of 6
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    Enhanced Aquaculture Wastewater Treatment Using Water Spinach (Ipomoea Aquatica Forsskal) and Exogenous Compound Bacteria
    (Elsevier, 2024) Xu, Jiaojiao; Nicholaus, Regan; Wang, Yangcai; Yang, Wen; Zhu, Jinyong; Zheng, Zhongming
    Bioremediation technology has been widely recognized as a sustainable treatment method for purifying aqua culture wastewater. We established two types of ecological floating beds, one with water spinach (WS) only and another with water spinach combined with exogenous compound bacteria (WS-ECB) for the treatment of the Pacific white shrimp aquaculture wastewater of low-salinity. The exogenous compound bacteria used in this study was composed of Rhodopseudomonas palustris and Bacillus subtilis in a certain proportion. Microbial com munity dynamics were analyzed by using high-throughput sequencing of 16S rRNA and water quality was determined. Both two treatments were effective in the remediation of aquaculture wastewater. The removal efficiencies of CODMn, TN and TP in the WS group were 34.33 %, 66.54 % and 73.01 %, respectively, and the removal efficiency of NH4 + in the WS-ECB group was 93.79 %. Furthermore, the addition of water spinach and exogenous compound bacteria increased the abundance of some bacteria that are involved in regulating water quality and altered the succession of microbial communities. The microbial communities were shaped by both stochastic processes (including limited dispersal) and deterministic processes (including environmental condi tions and biotic interactions). Importantly, stochastic processes dominated the assembly of both water and rhizosphere microbial communities in different treatments, while deterministic processes contributed more to the rhizosphere microbial community in the WS-ECB than in the WS. Overall, our study emphasizes that plant and microbial remediation was a potential and effective approach to remove the nutrients of water, and clarifies the bacterial community dynamics and assembly mechanisms during aquaculture wastewater treatment processes.
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    Nitrogen and Phosphorus Related Functional Genes Enhance Nutrient Removal in The Integrated Aquaculture Wastewater Bioremediation System in The Presence of Photosynthetic Bacteria.
    (Springer, 2024-12-20) Ramzan, Muhammad Naeem; Shen, Ding; Wei, Yingzhen; Emmanuel, Arslan; Nicholaus, Regan; Yang, Wen; Zheng, Zhongming
    Integrated Aquaculture Wastewater Bioremediation Systems (IAWBSs) are crucial for treating nutrient-rich mariculture water. However, there is a lack of detailed information about the functional mechanisms between nitrogen (N) and phosphorus (P) functional genes and the bacterioplankton community in the presence of photosynthetic bacteria (PSB). This study evaluated the connections between N and P functional genes in IAWBSs under the influence of PSB. The results showed significant improvements in effluent quality, with removal efficiencies of 79, 74, 83, 90, and 71% for NO2 −−N,NO3 −−N, PO4 3−-P, NH4 +−N, and CODmn, respectively. Adding PSB enhanced and altered microbial diversity within the system, promoting the abundance of functional genes related to N and P cycling. Notably, genes associated in denitrification (nirK and nirS), ammonification (ureC) and amoB, involved in nitrification, were significantly increased after PSB was added. Furthermore, genes such as phnK, phoD, and phoX, which are involved in P transformation, also showed increased abundance levels. These genes were closely linked to the microbial community distribution, species diversity, and nutrient cycling. Microbial community changes can result in changes in functional gene abundance. This study provides important and novel insights for developing bioremediation strategies for polluted sites. This demonstrates the fundamental relationships between the IAWBSs’ functional units and the distribution of microbial communities under the influence of PSB.
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    Nitrogen and Phosphorus‑Related Functional Genes Enhance Nutrient Removal in the Integrated Aquaculture Wastewater Bioremediation System in the Presence of Photosynthetic Bacteria
    (Springer nature, 2025) Ramzan, Muhammad N.; Shen, Ding; Wei, Yingzhen; Emmanuel, Arslan; Nicholaus, Regan; Yang, Wen; Zheng, Zhongming
    Integrated Aquaculture Wastewater Bioremediation Systems (IAWBSs) are crucial for treating nutrient-rich mariculture water. However, there is a lack of detailed information about the functional mechanisms between nitrogen (N) and phosphorus (P) functional genes and the bacterioplankton community in the presence of photosynthetic bacteria (PSB). This study evaluated the connections between N and P functional genes in IAWBSs under the influence of PSB. The results showed significant improvements in effluent qual- ity, with removal efficiencies of 79, 74, 83, 90, and 71% for NO2− −N, NO3− −N, PO43−-P, NH 4+ −N, and COD Mn, respectively. Adding PSB enhanced and altered microbial diversity within the system, promoting the abundance of functional genes related to N and P cycling. Notably, genes associated in denitrification (nirK and nirS), ammonification (ureC) and amoB, involved in nitrification, were significantly increased after PSB was added. Fur- thermore, genes such as phnK, phoD, and phoX, which are involved in P transformation, also showed increased abundance levels. These genes were closely linked to the micro- bial community distribution, species diversity, and nutrient cycling. Microbial community changes can result in changes in functional gene abundance. This study provides important and novel insights for developing bioremediation strategies for polluted sites. This demon- strates the fundamental relationships between the IAWBSs’ functional units and the distri- bution of microbial communities under the influence of PSB.
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    The Compound Bacillus and Sea Purslane (Sesuvium Portulacastrum) Enhanced Aquaculture Wastewater Treatment Efficiency: Insights from a Study on Microbial Community Distributions.
    (ELSEVIER, 2025-03-31) Hongmei Yuan; Li, Junxian; Wang, Haihang; Nicholaus, Regan; Ramzan, Muhammad Naeem; Yang, Wen; Zheng, Zhongming; Wang, Yangcai
    As the aquaculture industry continues to grow rapidly, the environmental consequences of wastewater discharge from aquaculture have become a major concern. The biological treatment method has proven to be an effective and beneficial approach for wastewater treatment. In this experiment, aquaculture wastewater was treated using two different ratios of Bacillus subtilis and Bacillus licheniformis composites in combination with sea purslane (Sesuvium portulacastrum) to investigate the wastewater treatment efficiency and microbial community. Sea purslane effectively removed nutrients from aquaculture wastewater. The addition of compound Bacillus significantly increased the removal rates of CODMn, TP, and PO4 3− -P, which were 54.9–56.4 %, 78.2–79.9 %, and 85.0–89.6 %, respectively. Moreover, the compound Bacillus notably influenced the microbial community composition in the water and root systems, reducing species abundance while increasing community diversity, with Treatment 2 exhibiting the highest community stability. The addition of the compound Bacillus significantly increased the relative abundance of Alphaproteobacteria. Most of the important operational taxonomic units (OTUs) that significantly impacted the groups belonged to Alphaproteobacteria. Different biomarkers in the water and root system were primarily from Alphaproteobacteria, Gammaproteobacteria, and Bacteroidia, including Idiomarina and Alteromonas, Ruegeria, Vibrio, Algoriphagus, and Maribacter, among others. These microorganisms play a significant role in promoting the removal rate of nitrogen and phosphorus. Furthermore, the compound Bacillus notably affected the abundance of nitrogen cycle-related sequences and increased the relative abundance of microorganisms associated with the nitrogen cycle. These findings provide valuable insights for optimizing aquaculture wastewater treatment processes.
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    The Functional Diversity of Microbial Communities in the Multi‑Compartment Biofilters of Shrimp Mariculture Effluents Using 16S rRNA Metabarcoding.
    (Springer, 2023-07-18) Lukwambe, Betina; Nicholous, Regan; Zheng, Zhongming
    Microbes play important roles in mariculture biofilter systems, such as biogeochemical cycling of nutrients and organic matter degradation. However, their functional diversity for nutrient removal in shrimp mariculture effluents influenced by multi-compartment biofilters remained elusive. This study explored the functional diversity of microbial communities for nutrient removal in the multi-compartment biofilters of shrimp mariculture effluents. We explored the bacterial taxonomy using Illumina sequencing of the 16S rRNA gene, and the ecological functional diversity of the bacteria was assigned using Functional-Annotation-of-Prokaryotic-Taxa analysis (FAPROTAX). According to the Illumina dataset, there was a high heterogeneity, with phase two (P2) treatment showing significant reversible shifts in microbial communities’ population and biogeochemical dynamics relative to phase one (P1). The genera Candidatus aquiluna, Marivita, HTCC , Anaerospora, and Arcobacter were abundant in both stages and may have the functional capability of biodegrading inorganic matter (OM). FAPROTAX results showed that the predominant functional groups (P1 and P2) for nutrient removal were related to chemo-heterotrophy. Furthermore, the nitrate-reduction and nitrate-ammonification were highly significant (P < 0.05) at P2, resulting in an average removal efficiency of 81.39% for total nitrogen (TN) and 80.63% for total phosphorus (TP). The results suggested that incorporating multicompartment biofilters in the system provides a suitable substrate environment for the potential proliferation of Proteobacteria, Nitrospirae, and Bacteroidetes. Overall, this study enlightens the potential roles of the microbial communities under the influence of biofilters in promoting the feasible and most efficient bioremediation approaches for mariculture effluents.
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    The Impact of Mariculture Biofilters on the Distribution of Benthic Nutrient Fluxes, Organic Matters and Bacterial Community in a Mariculture Wastewater Treatment System
    (Elsevier, 2024) Nicholaus, Regan; Lukwambe. Betina; Zheng, Zhongming
    Constructed-wetlands, biofilms, and sedimentation are among biological filters used in mariculture wastewater treatments, however, their impacts on the distribution of benthic microbial community and inorganic-nutrient fluxes have not been fully explored. This study applied 16 S rRNA high-throughput sequencing technology to investigate the microbial community distribution and their impacts on nutrient fluxes in mariculture biofilters system. Results showed that bacterial community compositions were significantly different in the constructed wetland and biofilm treatments (p < 0.05) relative to sedimentation. The composition of the 16 S rRNA genes among the treatments were enriched with Proteobacteria (73%), Bacteroidetes (69%), Firmicutes (62%), and Fla vobacteria (61%) in Biofilm compared to Proteobacteria (53%), Bacteroidetes (39%), Firmicutes (32%), and Fla vobacteria (21%) in constructed wetlands. NMDS analysis showed that bacterial composition in constructed wetland and biofilms clustered separately compared to sedimentation treatment. Functional-Annotation-of Prokaryotic-Taxa analysis indicated that the proportions of sediment-microbial-functional groups (aerobic-che moheterotrophy, chemoheterotrophy, and nitrate-ammonification) were 47% in the constructed-wetland, 32% in biofilm and 13% in sedimentation system. Benthic-nutrient fluxes for phosphate, ammonium, nitrite, nitrate and sediment oxygen consumption differed markedly among the treatments (p < 0.05). Canonical correspondence analysis indicated constructed-wetland had the strongest association between biogeochemical contents and the bacterial community relative to other treatments. This study suggests that the mariculture wastewater biofilters promoted microbial community distributions, sediment bacterial functional-groups including chemohetero trophy, aerobic-chemoheterotroph, denitrification, and nitrification and interactions with nutrient fluxes which was more pronounced in the constructed-wetland system.