The Functional Diversity of Microbial Communities in the Multi‑Compartment Biofilters of Shrimp Mariculture Effluents Using 16S rRNA Metabarcoding.
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Date
2023-07-18
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Springer
Abstract
Microbes play important roles in mariculture biofilter systems, such as biogeochemical cycling of nutrients and organic matter degradation. However, their functional diversity for nutrient removal in shrimp mariculture effluents influenced by multi-compartment biofilters remained elusive. This study explored the functional diversity of microbial communities for nutrient removal in the multi-compartment biofilters of shrimp mariculture effluents. We explored the bacterial taxonomy using Illumina sequencing of the 16S rRNA gene, and the ecological functional diversity of the bacteria was assigned using Functional-Annotation-of-Prokaryotic-Taxa analysis (FAPROTAX). According to the Illumina dataset, there was a high heterogeneity, with phase two (P2) treatment showing significant reversible shifts in microbial communities’ population and biogeochemical dynamics relative to phase one (P1). The genera Candidatus aquiluna, Marivita, HTCC , Anaerospora, and Arcobacter were abundant in both stages and may have the functional capability of biodegrading inorganic matter (OM). FAPROTAX results showed that the predominant functional groups (P1 and P2) for nutrient removal were related to chemo-heterotrophy.
Furthermore, the nitrate-reduction and nitrate-ammonification were highly significant (P < 0.05) at P2, resulting in an average removal efficiency of 81.39% for total nitrogen (TN) and 80.63% for total phosphorus (TP). The results suggested that incorporating multicompartment biofilters in the system provides a suitable substrate environment for the potential proliferation of Proteobacteria, Nitrospirae, and Bacteroidetes. Overall, this study enlightens the potential roles of the microbial communities under the influence of biofilters in promoting the feasible and most efficient bioremediation approaches for mariculture effluents.
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This Journal Article of Science and Technical Education was Published by Springer.