Halotolerant Pseudomonas koreensis S4T10 mitigate salt and drought stress in Arabidopsis thaliana
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Date
2023-11
Journal Title
Journal ISSN
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Publisher
Physiologia Plantarum
Abstract
Salt and drought are documented among the most detrimental and persistent abiotic
stresses for crop production. Here, we investigated the impact of Pseudomonas koreensis
strain S4T10 on plant performance under salt and drought stress. Arabidopsis thaliana
Col-0 wild type and atnced3 mutant plants were inoculated with P. koreensis or tap water
and exposed to NaCl (100 mM) for five days and drought stress by withholding water
for seven days. P. koreensis significantly enhanced plant biomass and photosynthetic pigments
under salt and drought stress conditions. Moreover, P. koreensis activated the antioxidant
defence by modulating glutathione (GSH), superoxide dismutase (SOD),
peroxidase (POD), and polyphenol oxidase (PPO) activities to scavenge the reactive oxygen
species produced due to the stress. In addition, the application of P. koreensis upregulated
the expression of genes associated with antioxidant responses, such as AtCAT1,
AtCAT3, and AtSOD. Similarly, genes linked to salt stress, such as AtSOS1, AtSOS2,
AtSOS3, AtNHX1, and AtHKT1, were also upregulated, affirming the positive role of
P. koreensis S4T10 in streamlining the cellular influx and efflux transport systems during
salt stress. Likewise, the PGPB inoculation was observed to regulate the expression of
drought-responsive genes AtDREB2A, AtDREB2B, and ABA-responsive genes AtAO3,
AtABA3 indicating that S4T10 enhanced drought tolerance via modulation of the ABA
pathway. The results of this study affirm that P. koreensis S4T10 could be further developed
as a biofertilizer to mitigate salt and drought stress at the same time.
Description
This article was published by Physiologia Plantarum in 2023